Steve Bruce's side return to action after a successful night in the League Cup in midweek, while Blackburn will be keen to climb towards mid-table after a disappointing run.
Hull should have no issues with confidence as they prepare for a return to the Championship after claiming a surprise win over Swansea City in the third round of the League Cup on Tuesday night.
It was further proof of the quality within the Hull squad, but any cup run will simply serve as a bonus to the club's supporters, who are desperate to see Bruce take the team back to the Premier League at the first attempt.
Hull have recovered from defeat to leaders Brighton & Hove Albion by beating Cardiff City and playing out a 1-1 draw with Queens Park Rangers last weekend, and the challenge for the squad is to now find their form on a consistent basis as they look to stay close to the top of the table.
With just Burnley, Middlesbrough and Brighton ahead of them at the moment, the early signs are promising for Bruce, who will be fully aware that not much can be taken from how the table looks in September.
Back-to-back league wins have evaded the Tigers so far this season, and Bruce must be hopeful that their impressive display in the League Cup will be enough to put them on course for an extended run of fine performances in the Championship.
Recent form: WLWLWD
Recent form (all competitions): WWLWDW
In truth, it was the type of performance which led so many people to be surprised by Blackburn's disappointing start to the campaign as they dominated from the first whistle to collect all three points against the Addicks with a brace from Jordan Rhodes and a late Tom Lawrence strike.
After being forced to deal with rumours which suggested that he could be on his way out of the club just a few weeks ago, some of the pressure was lifted from Bowyer's shoulders with the win and he will now be targeting successive victories as Rovers look to build on last season's ninth-place finish.
Some of the displays in the early weeks of the campaign must surely cast doubt on whether the squad is strong enough for a playoff push, but supporters will point to the improved form of Rhodes, who has netted four goals in the last three Championship games.
It took stubbornness from the board to keep the striker at the club, and if Blackburn continue to produce better performances in other areas of the pitch, Bowyer will be confident that Rhodes can make the team a force in the league.
Games against Hull and Ipswich Town will prove to be big tests, but renewed confidence in the camp ensures that the visitors are in good shape heading into Saturday's trip.
Recent form: DLDLDW
Blackburn are unlikely to make changes after recording their first win of the campaign, which means that Lawrence could be forced to settle for a spot on the bench following his impressive cameo last week.
Jake Livermore made his return to the Hull side in the victory over Swansea and the former Spurs midfielder is likely to be named on the bench for the first time in the Championship this season.
Hull possible starting lineup:
McGregor, Dawson, Bruce, Davies, Odubajo, Robertson, Huddlestone, Clucas, Diame, Akpom, Hernandez
Blackburn possible starting lineup:
Steele, Henley, Duffy, Hanley, Olsson, Marshall, Guthrie, Evans, Conway, Koita, Rhodes
Head To Head
The victory was Hull's only success in the last six league meetings between the two teams, with Blackburn claiming three wins and two draws from the other games.
We say: Hull 2-0 Blackburn
Blackburn will provide a tough test for Bruce's side after finally claiming a first win last weekend, but Hull should be fresh after a number of key players were rested in midweek. The opening goal is likely to be crucial, and if Hull can keep Rhodes quiet, they should manage to collect all three points.