Sassuolo, meanwhile, are outside contenders for a European spot following a particularly strong run of form since the Italian top flight returned to action.
Having been neck and neck with Juventus for the majority of the season before the coronavirus-enforced shutdown, Lazio fans would have no doubt believed that their side could be the ones to end the Old Lady's unparalleled dominance of the Italian football scene.
Juve's chances of securing their ninth title in a row were under serious threat until a couple of weeks ago, with Lazio drifting further away from Serie A glory after two successive losses to AC Milan and, most recently, relegation candidates Lecce on Tuesday.
Felipe Caicedo broke the deadlock after only five minutes for Simone Inzaghi's side in midweek, but Lecce hit back through Khouma Babacar and Fabio Lucioni to condemn Lazio to their third loss in five since the restart.
The challengers were unable to capitalise on Juve's 4-2 defeat to AC Milan and Lazio still lie seven points adrift of the champions after 31 matches, and it appears unlikely that Juve will relinquish their title now as they boast a healthy lead over Inzaghi's side with seven games remaining.
Lazio still have a fixture against the champions to contend with later in the month, but any more slip-ups for Inzaghi's men and the title race could already be done and dusted by the time the top two clubs lock horns.
Lazio's 3-0 loss to Milan last weekend was their first home defeat of the season, however, and I Biancocelesti have triumphed in 10 of their last 12 games on familiar territory.
With regards to Sassuolo, I Neroverdi did their European hopes the world of good as they enjoyed their third win in succession with a hard fought 2-1 triumph over Bologna in midweek.
Roberto De Zerbi's men now sit in eighth position - six points adrift of seventh-placed AC Milan - although Hellas Verona have the chance to leapfrog Sassuolo when they take on Inter Milan on Thursday evening.
Sassuolo enjoyed one stint in the Europa League during the 2016-17 campaign but finished rock bottom of their group that year, although a top-eight finish in the league would be a significant improvement on Sassuolo's bottom-half standings in the past three seasons.
The free-scoring Atalanta are the only other side that have tasted victory in their last three games in the top flight, and Sassuolo have only suffered defeat in three of their last 13 matches away from home.
Lazio Serie A form: WLWWLL
Sassuolo Serie A form: LDDWWW
Lazio defender Luiz Felipe is likely to be handed his first start since February as he replaces the suspended Patric, who received his marching orders for inexplicably biting Lecce's Giulio Donati in the dying embers of Tuesday's game.
Lazio possible starting lineup:
Strakosha; Felipe, Acerbi, Radu; Lazzarri, Milinkovic-Savic, Leiva, Alberto, Jony; Caicedo, Immobile
Sassuolo possible starting lineup:
Consigli; Muldur, Ferrari, Peluso, Kyriakopoulos; Locatelli, Magnanelli; Berardi, Djuricic, Boga; Caputo
We say: Lazio 2-1 Sassuolo
Sassuolo should feel confident of earning at least a point against a Lazio side struggling for form at the moment, but Inzaghi's men will be out to make a statement following their previous two defeats and we expect the home side to scrape a narrow victory and keep the pressure on Juventus.