Juve are yet to beat the Rome giants in three matches this season, which includes a Coppa Italia semi-final that saw Lazio edge through over two legs.
Petkovic has called on his team to focus on finishing strongly in Serie A following their Europa League exit to Fenerbahce last Thursday.
He told reporters at his pre-match press conference: "I congratulated the team because they gave 100% on Thursday. But we'll need to give 150% if we want to get a result tomorrow, otherwise it will be difficult.
"We'll be up against the strongest side in the division. However, Juventus aren't invincible and we've already overcome them in the Coppa Italia.
"I won't have a great deal of choice when it comes to my starting lineup, but all those who take to the field will give their all."