This will be the fifth time that the Blues have faced the Rovers on the final day of a league season, and they have yet to lose against them, winning and drawing two games each.
After winning 5-2 at home to Huddersfield Town at the end of April, Blackburn were unable to begin the month of May with another victory as they played out a 1-1 draw away at relegation-threatened Rotherham United last weekend.
Adam Armstrong capitalised on a defensive mistake from Angus MacDonald to slot home his 25th goal of the season 19 minutes in, but the hosts came out fighting in the second half and managed to score an equaliser four minutes from time through Lewis Wing, to see both teams share the spoils.
Blackburn claimed all three points against Birmingham in their most recent meeting earlier this campaign, with Armstrong and Bradley Dack scoring the goals to earn a 2-0 victory at St Andrews.
The Rovers, who have not lost at home in their final league match of a season since May 2000, are looking to complete a league double over the Blues for the first time since the 2000-01 campaign under former boss Graeme Souness.
Birmingham were also unable to begin the month of May with three points as they were thrashed 4-0 at home to Cardiff City last weekend.
A brilliant hat-trick from Harry Wilson, as well as a strike from Mark Harris, helped the Bluebirds claim an emphatic victory against a much-changed Birmingham outfit, who lost their 19th Championship game of the season.
Lee Bowyer's men are now 18th in the table, two places and two points better off than last season, and there are now no fears of relegation to the third tier.
Birmingham travel to Ewood Park having won just two of their last 34 away league visits against Blackburn, winning 2-1 back in 1968 and 3-2 in 2013.
The Blues have not finished higher than 17th in the last four Championship seasons, but a victory on Saturday could see them climb as high as 15th in the table.
With nothing to play for, Blackburn boss Mowbray may decide to make changes to the starting lineup that drew with Rotherham last weekend.
As many as nine other first team players, including Stewart Downing, Ryan Nyambe, Joe Rothwell and Bradley Johnson, will see their contracts expire next month and so could also be playing their final match for the club this weekend.
Armstrong, who has 25 Championship goals this season, is set to start up front and he is just two goals away from matching the tally of former Rovers striker Jordan Rhodes, who scored 27 times in 2012-13.
As for Birmingham, striker Scott Hogan has missed the last two matches with a hip injury and is unlikely to feature this weekend.
Youngsters Amari Miller and Ryan Stirk were handed their first senior starts in the defeat to Cardiff and the pair could play a part once again this weekend, as could Nico Gordon who came off the bench against the Bluebirds.
Blackburn Rovers possible starting lineup:
Kaminski; Nyambe, Lenihan, Harwood-Bellis, Douglas; Johnson, Rothwell, Holtby; Elliott, Armstrong, Gallagher
Birmingham City possible starting lineup:
Jeacock; Dacres-Cogley, Dean, Friend, Seddon; Stirk, Sunjic, Harper, Miller; Leko, Jutkiewicz
We say: Blackburn Rovers 2-1 Birmingham City
Both Blackburn and Birmingham can enjoy the final day of the season, with no pressure riding on the result.
The hosts are more likely to field a stronger side than their opponents and that could help them claim all three points at Ewood Park this weekend.