Union Berlin, meanwhile, still have a chance of competing in Europe themselves next season.
This time last month, Wolfsburg looked all but assured of competing in the Champions League next season for the first time since reaching the quarter-final in the 2015-16 campaign, but three defeats in their last four matches has seen fourth-placed Eintracht Frankfurt and fifth-placed Borussia Dortmund move one point and two points behind them respectively.
The Wolves' 2-0 defeat at home to Dortmund last time out was a particularly bitter blow, especially as BVB were reduced to ten men after Jude Bellingham's 60th-minute dismissal.
However, two superb strikes from Erling Braut Haaland either side of half time proved to be the difference between the two sides, with Oliver Glasner's men unusually lacking penetration. Indeed, despite having 21 shots across the match, Dortmund goalkeeper Marwin Hitz was only tested on two occasions.
Wolfsburg remain in the driving seat for a top-four place, but with tricky fixtures against in-form sides RB Leipzig and Mainz 05 to wrap up their season, they could really use a victory on Saturday to calm any nerves and strengthen their position in the table.
Union Berlin will certainly be no pushovers, however, with only the top four teams in the division having lost fewer matches than Urs Fischer's side this season.
They have European football of their own to fight for, too, given that they sit level on points with seventh-placed Borussia Monchengladbach.
Whoever occupies that position at the end of the season is likely to qualify for next season's inaugural UEFA Europa Conference League, although Bayer Leverkusen are only four points ahead of both sides in the second Europa League qualification spot.
Given that this is still only Union Berlin's second ever season in Germany's top flight, a European finish would be a remarkable achievement by Fischer and his players, although it would almost certainly necessitate adding greater squad depth for next season.
Based on their performance last time out against Werder Bremen, they would be good value for it, too, with Joel Pohjanpalo's clinical second-half hat-trick sealing a 3-1 victory. If Fischer's side can be as ruthless in both boxes as they have been for most of the season, there is every chance that they can upset the Wolves on Saturday.
Renato Steffen, who opened the scoring in a 2-2 draw in the reverse fixture, is unlikely to recover from his ligament injury in time to feature, but will be hoping to play another part in Wolfsburg's season yet.
Marin Pongracic is facing a race against time to play again this season due to his leg injury, while Bartosz Bialek suffered an ACL injury in training last week and as such could be out for the rest of the calendar year.
Glasner could be tempted to restore winger Josip Brekalo to his starting XI in order to retain more natural width, with Maximilian Philipp or Yannick Gerhardt potentially dropping out of the side as a result.
Union Berlin, meanwhile, were dealt a blow themselves after Cedric Teuchert was stretchered off due to a knee injury during the win against Bremen. The forward will miss the rest of the season.
Petar Musa will be on standby should Kruse miss out with a minor muscle issue, but Union Berlin's top goalscorer is likely to play through any pain for the cause.
Wolfsburg possible starting lineup:
Casteels; Mbabu, Lacroix, Brooks, Otavio; Schlager, Arnold; Baku, Gerhardt, Brekalo; Weghorst
Union Berlin possible starting lineup:
Luthe; Friedrich, Knoche, Schlotterbeck; Trimmel, Promel, Andrich, Ingvartsen, Lenz; Kruse, Pohjanpalo
We say: Wolfsburg 2-1 Union Berlin
We can envisage a narrow victory for the Wolves against a Union Berlin side which has proved tough to beat this season.
Glasner will be hoping Kruse does miss out with injury, as the visitors often lack creativity without their attacking talisman.