However, the Portuguese coach believes that his team can cope without the 23-year-old, who has scored 11 goals in his last 10 games.
"I will not rest anyone," Villas-Boas told reporters. "It is an important game for us and I will not take anything for granted. The result from the first leg is not enough.
"It is great for us to play big teams like Inter – we always put in the most amount of work possible, and we will continue to do so.
"This team does not need to prove anything – we have achieved significant results even without Gareth Bale."
The draw for the quarter-finals takes place in Nyon, Switzerland tomorrow.