The European tie is delicately poised following a goalless draw in the first leg at the Estadio Ramon last month.
Lenglet has said that Sevilla will "put everything" on the table as they bid to upset the Red Devils at Old Trafford.
"I believe we are ready for United," Lenglet told Cope Sevilla. "We are going to put everything into this game because of its significance but also because there is an international break coming up, so we will have an opportunity to recover after.
"We have put a lot of planning and preparation into this game with different strategies and ideas on how we can hurt them. We are a strong team once we take the lead and that is our aim."
United have only lost once at home in all competitions this season, but any score draw would see Sevilla advance to the quarter-finals of the Champions League.