The Tykes are bottom of the division, but can move level on points with Millwall with a win at the Madejski Stadium.
Peter Ramage underwent a scan on Monday, and it is unsure if the 30-year-old will be fit in time for the trip, while manager Danny Wilson revealed before the game that he is looking to bolster his squad through the loan market.
"We are looking (in the loan market). I wouldn't say it's desperate but it's very difficult to get the players in I think would help us," he told reporters.
"With what's available at the moment, there's a lot of young kids. You just don't know what you're getting. And then the experienced ones are being held on to."
Nigel Adkins's side are unbeaten in their last five games.
Possible starting lineups
Reading: McCarthy; Gunter, Obita, Gorkss, Pearce; Akpan, McAnuff, McCleary, McAnuff, Drenthe, Robson-Kanu
Barnsley: Steele; Hassell, Nyatanga, Kennedy, Hunt; Dawson, McLaughlin, Woods, Mellis, Jennings, O'Grady
Sports Mole says: 2-0