Despite their progress in the EFL Cup, the Tykes head to the Madejski Stadium on the back of a 1-0 defeat at Luton Town last weekend.
Although losing out at home to Luton came as a blow, Barnsley boss Gerhard Struber will still be encouraged by how his side have started the season.
Recording wins and clean sheets against Nottingham Forest and Middlesbrough in the EFL Cup have set up an opportunity to test themselves against Chelsea at Stamford Bridge.
While the game is lower down on their priority list, it is an opportunity for Struber to further assess the strength of his squad at a time when he is looking to create competition for places.
As many as 20 different players have already featured in their opening three matches, and Struber will hope that the desire to earn a starting spot against Chelsea will act as extra motivation this weekend.
Like the visitors, Reading have also suffered a 1-0 reverse at home to Luton over the past week, highlighting that those results should arguably be put into context.
However, the setback ended Veljko Paunovic's two-match winning streak since his arrival at the Royals, and the Serbian is now tasked with the job of ensuring that a second defeat is not suffered in the space of four days.
Although Paunovic made multiple changes to his winning team from the previous game, he must now decide whether his squad has enough depth to mount a bid for the playoffs.
Given his encouraging comments in the aftermath of the game versus the Hatters, it appears that he is willing to show faith in the players at his disposal.
Reading Championship form: W
Reading form (all competitions): WWL
Barnsley Championship form: L
Barnsley form (all competitions): WLW
Paunovic is expected to name the same starting XI which began the triumph in the East Midlands last weekend.
After missing parts of pre-season, Ovie Ejaria is nearing full match fitness after his 68-minute run-out against Derby.
Reading possible starting lineup:
Cabral; Yiadom, Morrison, Moore, Richards; Rinomhota, Laurent; Olise, Swift, Ejaria; Joao
Barnsley possible starting lineup:
Walton; Sollbauer, Andersen, Williams; Ludewig, Mowatt, Styles, Ritzmaier; Thomas; Schmidt, Woodrow
We say: Reading 1-2 Barnsley
Although Reading are the marginal favourites to prevail at the weekend, we cannot ignore Barnsley's form in the EFL Cup. Struber's side enjoy being the underdogs, and the visitors could live up that billing once again on Saturday.