The hosts are currently seventh in the division, level on points with sixth-placed Wolfsburg, while Union Berlin sit 12th with 38 points to show from their 32 matches this season.
Hoffenheim announced earlier this month that they had parted ways with head coach Alfred Schreuder with immediate effect, despite the fact that the club were in the latter stages of their Bundesliga campaign and searching for a top-six finish.
A team of coaches alongside director of football Alexander Rosen are leading the side in the latter stages of the season, and there is simply no downplaying the importance of this weekend's clash.
Die Kraichgauer bounced back from a home defeat against RB Leipzig by overcoming Augsburg on Wednesday night, which has left them in seventh position in the table.
They are level on points with sixth-placed Wolfsburg at this stage and will be determined to pick up a positive result this weekend considering that their final game of the season is away to Borussia Dortmund on June 27.
Union Berlin are competing in their first ever season at this level of football, and they can be extremely proud of their performances in the Bundesliga during the 2019-20 campaign.
A total of 38 points from 32 matches has left them in 12th position in the table, meaning that their spot in next season's top flight is already secure with two games left.
Die Eisernen were actually in deep trouble when they lost 4-1 at Borussia Monchengladbach at the end of May, but they have taken seven points from their last three matches, including back-to-back victories over Koln and Paderborn, to move out of trouble.
Urs Fischer's side will finish their season with a home game against Fortuna Dusseldorf on June 27, and the pressure is now off the players ahead of their final two matches.
Hoffenheim Bundesliga form: DWWDLW
Union Berlin Bundesliga form: LDLDWW
There are not expected to be many surprises from the side that started against Paderborn, though, with Sebastian Andersson again likely to lead the attack.
Hoffenheim possible starting lineup:
Baumann; Bosch, Grillitsch, Hubner; Kaderabek, Samassekou, Rudy, Baumgartner, Skov; Dabour, Larsen
Union Berlin possible starting lineup:
Gikiewicz; Trimmel, Friedrich, Schlotterbeck, Reichel; Ingvartsen, Gentner, Promel, Andrich, Bulter; Andersson
We say: Hoffenheim 2-1 Union Berlin
There is no question that it is a bigger match for Hoffenheim than it is for Union Berlin, who are already safe from the threat of relegation. The visitors are capable of pulling off a surprise, but we fancy Hoffenheim to put an important three points on the board as they battle for a top-six spot.