Chris Martin continues to lead the line for the hosts, who won this fixture 5-0 in the regular season, while Ayite and Johansen replace Lucas Piazon and Neeskens Kebano in the midfield alongside Kevin McDonald, Sone Aluko and captain Tom Cairney.
There is one change in the defence as Ryan Fredericks usurps Denis Odoi at right-back, with Tomas Kalas, Tim Ream and Scott Malone all keeping their places following their 2-1 victory at fellow promotion hopefuls Sheffield Wednesday on the last day of the regular season.
There is one change in the back five, meanwhile, as Tyler Blackett replaces Tiago Ilori alongside Chris Gunter, skipper Paul McShane - passed fit after a calf injury in the week - Liam Moore and Jordan Obita.
Fulham: Bettinelli; Fredericks, Kalas, Ream, Malone; Johansen, McDonald, Aluko, Cairney, Ayite; Martin
Subs: Button, Odoi, Sessegnon, Parker, Piazon, Kebano, Cyriac
Reading: Al-Habsi; Gunter, McShane, Moore, Blackett, Obita; Evans, Williams, Swift; Grabban, Kermorgant
Subs: Jaakkola, Van den Berg, Beerens, Mendes, McCleary, Ilori, Popa