The Blues currently lead the semi-final tie 1-0, meaning that they are not required to score in order to progress through to May's final in Munich.
However, Di Matteo believes that it would prove to be too dangerous if Chelsea decided to sit on their one-goal advantage.
"I do think we will have to try to score," Di Matteo told reporters. "It will be difficult to just play for a goalless draw.
"Barcelona will create chances against every team they play against us so we will have to try to score a goal but we have to also try to limit the chances they create against us."
Chelsea have not won in their last three trips to Barcelona.