To ensure progression, Wanderers manager Owen Coyle is expected to name a strong starting lineup for Macc's visit.
Macclesfield manager Gary Simpson rested a number of key players at the weekend with Tuesday's match in mind.
Bolton: Bogdan; Robinson, Wheater, Knight, Rickets; Eagles, Pratley, Reo-Coker, Petrov; Davies, Klasnic.
Macclesfield: Veiga; Bateson, Brisley, Brown, Tremarco; Hamshaw, Wedgebury, Mendy, Diagne, Daniel; Donnelly.
Sports Mole says: 3-1