Hansi Flick's side have won four major honours already this year, but they lost to Dortmund in this fixture last season and enter the game on the back of a heavy defeat.
This match will be staged behind closed doors, which is a first for the competition, due to the coronavirus infection rate in the state of Bavaria.
Just when it was looking as though Bayern were pretty much invincible, they were sent crashing back down to earth by Hoffenheim in a chastening defeat on Sunday.
Fatigue inevitably played a part in the 4-1 loss at the Rhein-Neckar-Arena, with Robert Lewandowski benched for a league game for the first time in two years.
That defeat ended Bayern's 32-match unbeaten run since last December, a streak that has seen them win the Bundesliga, DFB-Pokal, Champions League and UEFA Super Cup.
Flick will not be too downbeat, with the loss coming three days after being taken to extra time by Sevilla in the Super Cup, but his players have to quickly pick themselves up.
Dortmund also enter this match on the back of a league defeat at the weekend, going down 2-0 to Augsburg on Saturday in what was a hugely disappointing team display.
That was Dortmund's sixth triumph in this competition and ended a run of three successive victories for Bayern, who are the record seven-time winners and six-time runners-up.
Lucien Favre would of course love to see Lewandowski benched once again, but Flick is expected to go with his strongest possible XI on Wednesday, as will Dortmund.
Consistency is proving to be a major issue for Favre's men, having followed up each of their last three league victories with a defeat.
That has not been a problem for Bayern - the shock loss to Hoffenheim aside - and they will enter this one-off match as favourites to add yet another trophy to their collection.
Bayern Munich form (all competitions): WWL
Borussia Dortmund form (all competitions): WWL
Lewandowski is the DFL-Supercup's leading all-time scorer with five goals and will almost certainly replace Joshua Zirkzee in attack on Wednesday.
Leroy Sane has started all three matches for Bayern this season but picked up a knock at the weekend and is unlikely to play a full part here.
Erling Braut Haaland seems certain to retain his place in attack after scoring two goals in three outings so far this campaign, but Sancho has been ruled out through illness.
Bayern Munich possible starting lineup:
Neuer; Pavard, Sule, Alaba, Davies; Kimmich, Goretzka; Gnabry, Muller, Coman; Lewandowski
Borussia Dortmund possible starting lineup:
Burki; Can, Hummels, Akanji; Meunier, Brandt, Witsel, Guerreiro; Reyna, Reus; Haaland
We say: Bayern Munich 2-0 Borussia Dortmund
Bayern may have lost to Hoffenheim at the weekend, but that came on the back of a 23-match winning streak that included an 8-0 win over Schalke 04 in their previous league outing.
With star man Lewandowski restored to the starting lineup, we are tipping Flick's men to overcome Dortmund and gain revenge for their defeat in this fixture 13 months ago.