The Clarets have only ever scored seven Premier League goals against the Gunners and Barnes has netted three of those, so his absence could be keenly felt by Sean Dyche's side.
Indeed, Barnes is the only current Burnley player to have found the back of the net for the club against Arsenal, so the onus will be on the Clarets' fit strikers to improve that record.
Dyche will hand late fitness tests to three players as Johann Berg Gudmundsson, Robbie Brady and Jack Cork all push for inclusion, the latter having picked up a slight hamstring strain in the midweek draw with Leicester City.
Gudmundsson and Brady are now back in training and should be in contention to feature, but the quick turnaround in fixtures could lead to Cork missing out.
Changes are likely to be kept to a minimum elsewhere, although Erik Pieters is now back available and will be knocking on the door to start at left-back.
Burnley possible starting lineup: Pope; Lowton, Tarkowski, Mee, Taylor; Gudmundsson, Brownhill, Westwood, McNeil; Rodriguez, Wood
Watch the Sports Mole Football Shorts prediction for this game below: