Arsenal will have to overcome Barcelona across two legs in order to reach the quarter-finals of the Champions League.
The Gunners, who have not reached the quarter-finals since 2010, have faced the Catalan giants seven times, including the 2006 final which ended in a 2-1 defeat for Arsenal, and have won just once.
The last time that these two sides went head to head was in the 2010-11 campaign, when Arsenal sealed a 2-1 win in the home leg but were undone 3-1 by Barca at Camp Nou.
The first legs will be played on February 16/17 and 23/24, with the return legs taking place on March 8/9 and 15/16.
Champions League last 16 draw in full:
Gent vs. Wolfsburg
Roma vs. Real Madrid
Paris Saint-Germain vs. Chelsea
Arsenal vs. Barcelona
Juventus vs. Bayern Munich
PSV Eindhoven vs. Atletico Madrid
Benfica vs. Zenit St Petersburg
Dynamo Kyiv vs. Manchester City