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Rose and Kaymer had started well in moving one in front after three, but the American rookies began to warm to the task as they moved two ahead after seven.
However, Reed missed a chance at eight to extend the lead to three shots before he missed another putt at nine to allow Europe to reduce the deficit.
The young duo responded by winning the 10th, but Rose and Kaymer weren't finished and they took the 12th and 15th to level the match with three to play.
Reed missed a two-foot putt on 16 to miss a clear opening for USA to take the lead, but Europe slipped up on the penultimate green to allow the Americans to hold the momentum on the last.
Both teams sent their approaches into the greenside bunker, but it was Europe who played it better and Rose made no mistake from six feet to ensure that his team take a 10-6 lead into the singles.