The hosts are unbeaten in each of their last four matches on home soil, while the visitors are hoping to avoid a fourth successive away defeat.
After claiming a dramatic point in a 3-3 draw against Atletico Madrid with two stoppage time strikes, Valencia followed that up with a goalless draw away to high-flying Real Sociedad, who lost top spot in La Liga as a result.
The hosts were reduced to 10 men when Aritz Elustondo lashed out at Daniel Wass in the 76th minute, however, Jose Bordalas's men were unable to find a winner, even with the extra man, and were forced to share the spoils for the sixth time this season.
That result sees Los Che remain just inside the top 10, but they are now five points behind Saturday's opponents Rayo Vallecano in sixth.
Valencia made a bright start to the campaign after claiming 10 points from their first 12 on offer, however since then, they have accumulated just eight points from a possible 30 available.
Los Che have only won one of their last five meetings against Rayo Vallecano, though they did win the most recent encounter on home soil, securing a 3-0 victory in November last year.
Rayo Vallecano's surprisingly bright start to the campaign continued on Monday, with a 3-1 victory at home against Mallorca.
Goals from Sergio Guardiola, Alvaro Garcia and Oscar Trejo helped Los Franjirrojos secure their seventh league win of the season and keeps the newly-promoted outfit just three points behind the top four.
Rayo have collected 23 points from their first 14 La Liga matches this season, which is their joint-highest tally at this stage of a top-flight campaign, matching their efforts from 2000-01.
Andoni Iraola will be delighted with his side's impressive start, though their form away from home is something which needs addressing after losing each of their last three on the road.
Rayo did, however, claim a 2-0 in their last encounter against Valencia in April 2019, so that should provide them with confidence of securing another positive result this weekend.
Top scorers Carlos Soler and Hugo Duro – who have netted four goals each this term – are set to continue on the flanks, while forward Goncalo Guedes will be hoping to end a five-game drought without a goal.
After recovering from a groin problem, Radamel Falcao returned to the bench but was an unused substitute last weekend. The 35-year-old will now be hoping he can force his way into the first XI ahead of Guardiola.
Valencia possible starting lineup:
Cillessen; Foulquier, Diakhaby, Alderete, Gaya; Soler, Wass, Guillamon, Duro; Guedes, Gomez
Rayo Vallecano possible starting lineup:
Dimitrievski; Balliu, Saveljich, Catena, F. Garcia; Ciss, Comesana; Palazon, Trejo, A. Garcia; Guardiola
We say: Valencia 1-1 Rayo Vallecano
Both teams will feel that they are more than capable of claiming all three points from this fixture as they aim to climb further up the La Liga table.
However, with little to separate these two top-10 sides, a score draw could be on the cards at the Mestalla.