Andre Villas-Boas's side have already secured top spot in Group K thanks to a perfect record of five wins from their opening five matches.
Meanwhile, Russian visitors Anzhi are also through to the last 32 as they sit five points clear of second-placed Sheriff Tiraspol heading into their final fixture.
The hosts are likely to rest a number of key players, while Vlad Chiriches and Jan Vertonghen are expected to miss out through injury. Ali Gadzhibekov is absent for the away side with a fractured jaw.
Possible starting lineups
Spurs: Friedel; Naughton, Dawson, Capoue, Walker; Lennon, Dembele, Capoue, Sandro, Lamela; Soldado
Anzhi: Kerzhakov; Angbwa, Gadzhibekov, Ewerton, Grigalava; Eshchenko, Jucilei, Epureanu, Burmistrov; Maksimov, Serderov
Sports Mole says: 2-0