Julen Lopetegui's side booked their spot in the semi-finals of the Copa del Rey with a 1-0 victory over Almeria on Tuesday, while Getafe will enter the clash off the back of a goalless draw with Alaves in the league.
Lucas Ocampos scored the only goal of the contest on Tuesday night as Sevilla recorded a 1-0 win over second-tier side Almeria to advance to the semi-finals of the Copa del Rey.
Lopetegui's side are entering a vital stage of the season as they will also take on Borussia Dortmund in the last-16 stage of the Champions League, with the first leg to be held on February 17.
The Europa League holders are also chasing down second-placed Barcelona in Spain's top flight as they sit in fourth position in the table, just one point behind Ronald Koeman's side on the same number of matches.
Sevilla are four points clear of fifth-placed Villarreal with a game in hand, meanwhile, meaning that they are in pole position to secure another top-four finish, which would not be a surprise.
Los Nervionenses have been victorious in each of their last three in Spain's top flight, including a 2-0 success at Eibar last weekend, with Ocampos and Joan Jordan on the scoresheet for the visitors.
Getafe, meanwhile, were held to a goalless draw by Alaves last weekend, with the result leaving the capital side in 12th position in the table, six points off the top seven heading into the next set of fixtures.
Jose Bordalas's side, who finished eighth in La Liga last season, are entering a tough period of matches as they will face Sevilla, Real Madrid and Real Sociedad in their next three in Spain's top flight.
The Madrid club have struggled for consistency during the 2020-21 campaign, winning just six of their 20 matches and suffering eight defeats in the process, while they have only scored 17 league goals, which is the joint-second worst record in Spain's top flight this term.
Getafe's away form will also be a concern heading into this match, with Bordalas's team picking up just nine points from their nine fixtures on their travels, recording just two victories and suffering four defeats.
Sevilla La Liga form: DWLWWW
Sevilla form (all competitions): WWWWWW
Getafe La Liga form: LLWWLD
Getafe form (all competitions): LLWWLD
Acuna's absence should see Karim Rekik continue at left-back, while Ocampos's importance for the Europa League winners means that the Argentina is unlikely to be rested.
Getafe, meanwhile, will be without the services of Mauro Arambarri due to the booking that the midfielder picked up in the goalless draw with Alaves last weekend.
Arambarri's absence could see Francisco Portillo earn a staring role, but there are unlikely to be any changes in the final third with Carles Alena and Takefusa Kubo set to join Jaime Mata in a front three.
Sevilla possible starting lineup:
Bounou; Navas, Kounde, Gomez, Rekik; Torres, Fernando, Jordan; Suso, En-Nesyri, Ocampos
Getafe possible starting lineup:
Yanez; Chakla, Etxeita, Timor; Suarez, Portillo, Maksimovic, Cucurella; Kubo, Mata, Alena
We say: Sevilla 2-0 Getafe
Getafe are certainly capable of causing Sevilla problems this weekend, but it is very difficult to look past Lopetegui's side considering their form. The visitors will have second-placed Barca in their sights, and we fully expect Los Nervionenses to continue their winning run on Saturday night.