Defeat for Scotland would end their hopes of making the 2014 tournament in Brazil, as they currently sit 11 points behind group leaders Belgium.
Serbia come into the match on the back of three defeats in Group A, having lost 2-0 in Croatia on Friday.
When the sides met at Hampden Park last year, the game finished goalless.
Possible starting lineups
Serbia: Brkic, Ivanovic, Subotic, Nastasic, Kolarov, Radovanovic, Petrovic, Tosic, Stevanovic, Scepovic, Tadic
Scotland: McGregor, Hutton, Mulgrew, Hanley, Cadlwell, Dorrans, Adam, McArthur, Maloney, Burke, Rhodes
Sports Mole says: 1-1