The Italian outfit, who are featuring in this competition for just the second time in their history, will be unable to call upon Remo Freuler in this match, with the 28-year-old suspended due to the red card that he picked up in his side's one-goal loss to Madrid in the first leg of the last-16 tie.
Atalanta will also be without Hans Hateboer due to a foot injury, but the Serie A outfit did not pick up any fresh concerns in their 3-1 win over Spezia on Friday.
Head coach Gian Piero Gasperini is not expected to spring any real surprises with his team selection on Tuesday, meaning that the visitors should line up in a 3-4-1-2 formation, with Luis Muriel and Duvan Zapata operating in the final third of the field.
Cristian Romero was left out of the starting side on Friday but should return to the back three, while Pierluigi Gollini has started the last two Champions League games and is likely to feature between the sticks.
Robin Gosens has been in impressive form for Atalanta this season, scoring 10 times and registering seven assists in 33 outings, and the 26-year-old will again feature as the left-sided wing-back.
Atalanta BC possible starting lineup: Gollini; Toloi, Romero, Djimsiti; Maehle, De Roon, Pasalic, Gosens; Pessina; Muriel, Zapata