The Liverpool-born coach will come face-to-face with the former Championship club that sacked him in January, with the Royals fighting to move from the bottom of the table in the Premier League.
The Saints, who are now led by Mauricio Pochettino, are in a similar, yet not as serious, predicament as Saturday's opponents, as they reside four points away from the drop zone with seven games left to play.
Adkins was able to steer a slender 1-0 victory earlier on in the season over Reading while he was at the helm of Southampton, and he will have to repeat that feat on the other side following the Royals' run of consecutive defeats.
Neither side are expected to make drastic changes to their teams from last weekend.
Possible starting lineups
Reading: Taylor; Kelly, Pearce, Mariappa, Shorey; Leigertwood, Karacan, Guthrie, McAnuff, Robson-Kanu; Pogrebnyak
Southampton: Boruc; Clyne, Yoshida, Hooiveld, Shaw; Puncheon, Schneiderlin, Cork, Davis, Rodriguez; Lambert
Sports Mole says: 1-1