The 30-year-old suffered an injury to his right thigh during training this week, and will be sidelined for around 20 days.
"Fabio Quagliarella became the victim of an injury," read a statement from the club.
"The player underwent examinations which revealed a first-grade lesion to the long adductor in his right thigh. The prognosis is 20 days."
Quagliarella is certain to miss Juventus's visit to Fiorentina this weekend, as well as next Wednesday's Champions League clash with Real Madrid.
He is also doubtful for the Serie A meetings with Genoa, Catania and Parma.