Only three points separate the teams in the table heading into the penultimate round of fixtures, with Fiorentina in 12th while Bologna lie 10th after 36 matches.
Fiorentina's six-game unbeaten run in the Italian top flight ended in disappointing and controversial fashion at the hands of Roma on Sunday evening.
La Viola had enjoyed three victories and three draws from their last six outings prior to the weekend loss to Roma, and a three-point gap currently separates Fiorentina from the top half of the table with just two games remaining.
It seemed unfathomable for a club of Fiorentina's stature to suffer relegation from Serie A, and while La Viola have comfortably secured their safety in the top flight, Giuseppe Iachini knows that there is work to be done if Fiorentina are to mount a charge for a top-six finish next term.
In contrast, Bologna managed to end a five-game winless streak at the weekend as they came out on the right end of the scoreline in a five-goal thriller with Lecce.
Sinisa Mihajlovic's men left it late to claim all three points, however, with Musa Barrow's winner three minutes into added time ensuring that Bologna took the spoils in a 3-2 victory against the relegation candidates.
As is the case with Fiorentina, Bologna are firmly in mid-table obscurity and there is little riding on these final two games for Mihajlovic, and I Rossoblu have a relatively friendly final-day fixture with Torino to look forward to next weekend.
Bologna will no doubt want to try and better last season's 10th-placed finish, though, and Mihajlovic's men only lie behind ninth-placed Hellas Verona on goal difference before the penultimate round of matches.
The points have been shared in the last three meetings between Fiorentina and Bologna, although Fiorentina have not tasted defeat against I Rossoblu since the 2012-13 campaign.
Fiorentina Serie A form: DDWWDL
Bologna Serie A form: LDDLLW
Fiorentina have no fresh injury or suspension concerns heading into the midweek clash with Bologna.
As for Bologna, Rodrigo Palacio picked up his fifth booking of the season in the victory over Lecce and will sit this one out.
The Argentine's absence could see Barrow shifted to a central role, allowing Nicola Sansone to come back in on the left-hand side.
Fiorentina possible starting lineup:
Terracciano; Milenkovic, Pezzella, Caceres; Chiesa, Pulgar, Castrovilli, Ghezzal, Dalbert; Ribery, Cutrone
Bologna possible starting lineup:
Skorupski; Mbaye, Danilo, Denswil, Krejci; Medel, Svanberg; Orsolini, Soriano, Sansone; Barrow
We say: Fiorentina 2-1 Bologna
Both Fiorentina and Bologna will already be looking ahead to next season and have nothing to fear heading into the final stretch. That being said, Fiorentina boast a strong recent record against Bologna and were unfortunate to come away from their meeting with Roma empty-handed, so we are going for a narrow home victory.