The Belgian has been out of action for the Blues since the quarter-final second leg against Paris Saint-Germain earlier this month.
Hazard's return is one of three changes made by Chelsea from last week's goalless first leg in the Spanish capital.
Visitors Atletico, meanwhile, have also made three changes to the side that were held by Mourinho's men last week.
Chelsea: Schwarzer; Ivanovic, Cahill, Terry, Cole; David Luiz, Ramires; Azpilicueta, Willian, Hazard; Torres
Subs: Hilario, Kalas, Oscar, Van Ginkel, Schurrle, Ba, Eto'o
Atletico: Courtois; Juanfran, Miranda, Godin, Filipe Luis; Mario Suarez, Tiago; Adrian, Koke, Arda; Diego Costa
Subs: Aranzubia, Alderweireld, Garcia, Rodriguez, Jose Sosa, Diego, Villa
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