As a result, the loser of this tie is likely to face elimination from the competition at the group stages with just one more match remaining.
Any success that Juve are able to achieve will have to be done without the services of the striker Mirko Vucinic, who is struggling with a knee problem.
However, Andrea Pirlo is expected to return to their starting lineup following a domestic suspension. Pirlo's inclusion is likely to see former Manchester United youngster Paul Pogba drop down to the bench.
Chelsea's major selection dilemma surrounds the centre-forward position. Head coach Roberto Di Matteo has hinted that he is considering dropping Fernando Torres, with Daniel Sturridge tipped to benefit.
Juve have won 12 of their last 20 meetings against English opposition, although they have only emerged victorious from just one of the previous six.
Meanwhile, the Blues have only won once from eight visits to Italy.
Possible starting lineups:
Juventus: Buffon; Barzagli, Bonucci, Chiellini; Lichtsteiner, Marchisio, Pirlo, Vidal, Isla; Giovinco; Quagliarella
Chelsea: Cech; Ivanovic, Cahill, Luiz, Cole; Mikel, Ramires, Mata, Oscar, Hazard; Sturridge
Sports Mole says: 1-1