The full-back has been out of action for most of November because of a hamstring injury.
However, he was deemed fit enough for a place on the bench during Saturday's 2-1 loss to West Bromwich Albion and is now expected to start against the Italian champions.
"Ashley Cole is available to play. The defensive focus of the team will be very important tomorrow," Di Matteo told reporters.
Cole's inclusion is likely to mean that Ryan Bertrand will dropped down to the bench.