Huddersfield have lost their appeal against striker Steve Mounie's dismissal in Saturday's home Premier League defeat to Brighton.
Mounie was shown a controversial straight red card by referee Michael Oliver in the 32nd minute for his challenge on Brighton midfielder Yves Bissouma.
"Huddersfield Town's appeal against the red card shown to striker Steve Mounie in the game against Brighton & Hove Albion on Saturday, December 1 2018 has been unsuccessful," the club said on their official website.
Town boss David Wagner was furious at a number of key decisions made by Oliver during Saturday's game.
The Terriers had led through Mathias Jorgensen's first-minute header, but Brighton hit back after Mounie's dismissal to win 2-1 courtesy of goals from Shane Duffy and Florin Andone.
"After Town submitted the appeal on Monday, the regulatory commission informed the club that the appeal had been unsuccessful, meaning Steve will now serve a three-match suspension," Huddersfield added.
"This rules the striker out of the upcoming Premier League games against AFC Bournemouth (A) on December, 4 Arsenal (A) on December 8 and Newcastle United (H) on December 15."
Laurent Depoitre is set to return to the starting line-up in Mounie's absence for Tuesday night's Premier League game at Bournemouth.
Wagner, who has no new injury worries for the trip to the south coast, was also aggrieved by Oliver and his assistants' refusal to award Town a first-half penalty against Brighton after Alex Pritchard appeared to have been hauled down in the box.
Another contentious decision saw Brighton substitute Leon Balogun receive a yellow card for his tackle on Erik Durm in the closing stages.
"Sometimes situations like this make you stronger and give you more desire," Wagner said. "This wasn't what we deserved, but it's in the past and now it's about the next challenge.
"It's a big game (at Bournemouth) because after the experience we had two days ago we want to get points on the board. It's a very difficult task.
"Bournemouth have a lot of qualities. We want to make sure we are ready and we will see what we get out of this game. We have to be at our best."
Meanwhile, the Terriers manager fully endorsed the club's response to reports of homophobic chanting during Saturday's defeat to Brighton.
Huddersfield launched an investigation after their LGBT supporters group, Proud Terriers, claimed homophobic abuse was aimed at Brighton's travelling fans.
Huddersfield said any of their fans found to have been involved in the abuse will be banned from the John Smith's Stadium.
"I hadn't heard it during the game, but I'm aware of the reports and we will be very strong with it and make sure no one is welcome who has said this," Wagner added. "We will deal with it very strongly."