The Spanish champions will enter this weekend's clash off the back of a 1-0 win over Espanyol on Wednesday, while Valladolid suffered a 2-1 defeat at Valencia 24 hours earlier.
Valladolid finished 16th in La Liga last season but are heading for an improvement on that position this term as they currently sit 14th in the division with three games left.
All in all, it has been a decent campaign for the White and Violets, who are 10 points clear of the relegation zone and actually only four points off 12th-placed Levante.
Pucela have not finished higher than 14th in Spain's top flight since 2001-02, but they will fancy their chances of picking up points in their final two games of the campaign against Eibar and Real Betis.
Valladolid will enter this weekend's match off the back of a 2-1 defeat to Valencia, but they had been on a four-game unbeaten run before the clash with Los Che at the Mestalla.
Sergio Gonzalez's side have lost each of their last three league matches with Barca, although they did record a 1-0 victory over the Catalan giants on home soil back in March 2014.
As it stands, Barca are second in La Liga, just a point off leaders Real Madrid, having picked up a vital win against bitter rivals Espanyol on Wednesday night.
Zinedine Zidane's side will have the chance to again move four points clear when they play their game in hand against Alaves on Friday, though, and it would be a surprise to see the capital side fail to triumph.
Barca will surely have to win their final three games of the season against Valladolid, Osasuna and Alaves to stand a chance of finishing top of the pile.
The Catalan giants have not actually lost in the league since Real Madrid beat them at the start of March, but they have drawn three of their last six in La Liga, which has seen them relinquish control of first spot.
Quique Setien's side will be the favourites to triumph this weekend, but they have lost five of their 17 away league games this term, recording just seven wins in the process.
Valladolid La Liga form: LDDDWL
Barcelona La Liga form: DWDDWW
Valladolid will have Oscar Plano back from suspension this weekend, but a relatively lengthy injury list includes Jose Antonio Caro, Michel Javaloyas, Miguel de la Fuente, Matheus Fernandes and Pedro Porro.
Samuel Umtiti and Junior Firpo remain doubts with knee and hip problems respectively, but Frenkie de Jong, who has been on the sidelines since the middle of June with a calf injury, has returned to training and should be fit to return in the middle of the park.
Valladolid possible starting lineup:
Masip; Antonito, Olivas, Salisu, Martinez; Fede, Perez; Plano, Hervias, Guardiola; Ramirez
Barcelona possible starting lineup:
Ter Stegen; Roberto, Pique, Lenglet, Alba; De Jong, Busquets, Vidal; Griezmann, Suarez, Messi
We say: Valladolid 0-2 Barcelona
Only a win will do for Barca as they chase down Real Madrid, and we are finding it difficult to see the champions slipping up here. De Jong's expected return is excellent news for the Catalan giants, who should have too much for the home side on Saturday night.