The Spanish giants were the first team to confirm their place in the last 16 of the competition and know that victory would clinch first place with Inter Milan taking on Tottenham Hotspur in the group's other game.
However, Ernesto Valverde's side go into the game winless in their last three matches across all competitions and having been knocked off top spot in La Liga at the weekend.
Here, Sports Mole looks at how Barca could line up for Wednesday's match.
Barcelona were hit with a double injury blow at the weekend as Sergi Roberto and Rafinha both suffered problems which will rule them out of this match.
Roberto may be sidelined for the remainder of 2018, while Rafinha's ACL injury is likely to keep him out for the rest of the season.
The duo join a growing injury list for Barcelona, who are also missing Luis Suarez, Arthur, Jasper Cillessen and Thomas Vermaelen for the trip to Eindhoven.
Doubts remain over the fitness of Philippe Coutinho too, but Ivan Rakitic is available to bolster the midfield once again having served a suspension against Atletico Madrid on Saturday.