Inter Milan's Mauro Icardi has made the selection following an impressive 2017-18 campaign, but it is thought that the forward is at risk of being cut from the final squad at the start of June.
Juventus's Paulo Dybala is also included alongside Barcelona forward Lionel Messi, while there are seven Premier League players in the squad - including Manchester United pair Sergio Romero and Marcos Rojo, and West Ham United attacker Manuel Lanzini.
Argentina will open their 2018 World Cup campaign against Iceland on June 14, while they will also take on Croatia and Nigeria in the group stage of the competition.
Goalkeepers: Romero, Guzman, Caballero, Armani
Defenders: Mercado, Otamendi, Fazio, Tagliafico, Rojo, Acuna, Funes Mori, Ansaldi, Salvio, Pezzella
Midfielders: Mascherano, Di Maria, Banega, Biglia, Lanzini, Lo Celso, Centurion, Guido Pizarro, Paredes, Meza, Enzo Perez, Pablo Perez, Battaglia
Forwards: Messi, Aguero, Higuain, Dybala, Icardi, Pavon, Martinez, Perotti