Valverde can still call upon star man Messi, who will start alongside returning academy product Deulofeu and ex-Valencia attacker Alcacer.
The visitors, without a win against Barcelona in 12 attempts, have a number of new arrivals in their squad, though ex-Barcelona ace Cristian Tello is absent through injury.
Victor Camarasa, Sergio Leon, Zouhair Feddal, Andres Guardado, Juan Jose Narvaez and Antonio Barragan all come in from the off for the Verderones, who are aiming to better last season's 12th-place finish in the Spanish top flight.
Barcelona: Ter Stegen; Semedo, Mascherano, Umtiti, Alba; Busquets, Rakitic, Roberto; Messi, Deulofeu, Alcacer
Subs: Cillessen, Pique, Denis Suarez, Turan, Digne, Andre Gomes, Aleix Vidal
Real Betis: Adan; Barragan, Mandi, Feddal, Tosca; Guardado, Camarasa, Narvaez, Joaquin, Nahue; Sergio Leon
Subs: Gimenez, Jordi Amat, Durmisi, Javi Garcia, Fabian, Sanabria
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