Kane has reportedly asked to leave the club this summer but interim boss Ryan Mason has confirmed he will start against Villa.
Tyrone Mings' knee injury will be assessed after he missed Villa's 3-2 defeat at Crystal Palace on Sunday.
Midfielder Morgan Sanson will sit out the final two games of the season after suffering a reoccurrence of his knee problem.
Tottenham provisional squad: Lloris, Hart, Aurier, Doherty, Tanganga, Dier, Alderweireld, Rodon, Sanchez, Reguilon, Hojbjerg, Winks, Sissoko, Ndombele, Lo Celso, Alli, Lamela, Moura, Kane, Vinicius, Bale, Son.
Aston Villa provisional squad: Martinez, Heaton, Steer, Taylor, Engels, Elmohamady, Hause, Mings, Konsa, Targett, Barkley, Chukwuemeka, El Ghazi, Luiz, McGinn, Nakamba, Ramsey, Grealish, Traore, Watkins, Davis, Wesley.