Coming into just their second European Championship since independence, Stefan Tarkovic's squad contains several veteran components, but also some emerging talents who may also feature in their opening Group E match.
This week, Tarkovic was pleased to receive clearance for veteran playmaker Marek Hamsik to return from his calf injury, sustained while on a short-term contract at IFK Goteburg at the end of last season.
The Falcons' captain will not be joined by long-time national skipper Martin Skrtel at these Euros, however, after Tarkovic failed to convince the ex-Liverpool centre-back to end his international retirement. Peter Pekarik will therefore start, after the squad's most experienced defender earned his 100th cap in last week's friendly against Bulgaria.
Only versatile Slavia Prague forward Ivan Schranz is set to be missing on Slovakia's opening night, as he suffered an unfortunate thigh injury while leading the line against Austria during their final warm-up friendly.
Twenty-one-year-old striker Robert Bozenik is set to feature from the bench after playing 16 times for Feyenoord this term, while teenage midfielder Tomas Suslov - also based in the Eredivisie, with Groningen - is an exciting possible second-half replacement.
Slovakia possible starting lineup: Dubravka; Pekarik, Satka, Skriniar, Hubocan; Kucka, Benes; Haraslin, Hamsik, Mak; Duda