James Rodriguez, who moved to the Bernabeu from Monaco for a reported £64m fee, opened his account for the Spanish giants by finding the back of the net on Tuesday in the 81st minute.
The Ballon d'Or winner was forced off the field at half time on Tuesday with a leg problem, but manager Carlo Ancelotti has played down the severity of the injury.
Angel di Maria and Sami Khedira are unlikely to feature in the starting lineup after Ancelotti confirmed that the pair want to leave the Bernabeu this summer, but new recruit Toni Kroos is expected to play.
Atletico are expected to field Mario Mandzukic up front, while first-leg goalscorer Garcia should keep his place in the team for the clash on home turf.
Atletico Madrid: D
Real Madrid: D
Possible starting lineups
Atletico Madrid: Moya; Juanfran, Miranda, Godin, Siqueira; Mario, Gabi, Koke, Saul; Raul Garcia, Mandzukic
Real Madrid: Casillas; Carvajal, Pepe, Ramos, Marcelo; Modric, Kroos, Alonso; Bale, Ronaldo, Benzema
Sports Mole says: 1-2 (3-2 Real Madrid on aggregate)