Middleton cannot face his parent club under the terms of the agreement.
On-loan Rangers winger Glenn Middleton misses out for St Johnstone when his parent club visit Perth.
Saints boss Callum Davidson reported a couple of injury doubts but did no elaborate on names.
Midfielder Murray Davidson is still working his way back from a calf injury which he picked up last time out against Rangers.
Rangers could hand skipper James Tavernier a return to action after he returned to the bench on Sunday following a knee problem.
He could replace Nathan Patterson who, along with team-mates Bongani Zungu and Calvin Bassey, is waiting on news over the club's appeal against immediate four-match bans for breaching lockdown rules.
Ryan Jack (calf) and Niko Katic (knee) are out for the season while Leon Balogun (Achilles) is also missing.