Lazio's hopes of forcing their way into contention for one of Serie A's European berths were dented this evening courtesy of Sassuolo, who claimed all three points at the Stadio Olimpico thanks to goals from Domenico Berardi and Gregoire Defrel.
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Sassuolo, meanwhile, are now four points ahead of their hosts following their second win on the bounce.
The deadlock was broken in Rome four minutes before the break when Lazio's Mauricio was penalised for a foul inside the area and Berardi stepped up to fire in his sixth goal of the campaign from the spot.
Playing on the counter-attack after the restart, the away side went on to double their advantage in the 67th minute.