The Azzurri will confirm their spot in next summer's South American showpiece with maximum points against the Czechs after edging Bulgaria 1-0 in Palermo last Friday.
Prandelli has conceded that he expected a much sterner test in a group featuring Denmark and Armenia, but remains adamant that he respects the Czechs and expects a tough test in Turin this evening.
He told reporters: "This is an important game for us, we know that a good result will see us at the World Cup.
"We have great respect for the opponents though. We know they can attack the spaces well and have a midfield of excellent quality. We'll have to be focused for the entire game.
"We never thought we'd be in this position in the group at the start of the campaign. The Eastern teams are a lot stronger than they were a few years ago. But we have built this qualification on consistency."