Real Madrid midfielder Luka Modric and Juventus striker Mario Mandzukic are both missing out through injury, so there are starts for 20-year-old midfielder Marko Rog and 21-year-old forward Marko Pjaca.
Spain boss Vicente del Bosque, meanwhile, resists the temptation to make changes, so his starting XI is the same lineup that has started both of their Group D games at this summer's competition.
Alvaro Morata, who has just re-signed for Real Madrid, leads the attack with support from in-form Nolito and David Silva, while Cesc Fabregas also keeps his spot in the XI despite calls for Koke to be included.
Croatia: Subasic; Srna, Corluka, Jedvaj, Vrsaljko; Perisic, Rog, Rakitic, Badelj; Pjaca, Kalinic
Spain: De Gea; Juanfran, Pique, Ramos, Alba; Busquets, Fabregas, Iniesta; Silva, Morata, Nolito
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