Coronavirus restrictions over travel mean that this fixture cannot take place in Spain, and as a result, Atletico's home leg against Thomas Tuchel's side will take place in Romania.
Here, Sports Mole looks at how Atletico could line up against the Blues.
Atletico will again be without the services of Hector Herrera and Kieran Trippier through coronavirus and suspension respectively, while three first-team players are unavailable through injury.
Neither Yannick Carrasco nor Sime Vrsaljko have been able to recover from their problems to be included in the squad, while Jose Gimenez suffered a muscular problem against Levante and has also been ruled out.
Gimenez's place at the back should be taken by Stefan Savic, who was suspended in La Liga on Saturday, with Simeone widely expected to stick with a back three for Tuesday's European contest.
Moussa Dembele made his debut on Saturday, having recovered from coronavirus, and the 24-year-old, who is on loan from Lyon, has been named in the travelling squad.