The Premier League giants will take on the Italian champions in Turin in Group E.
"It's going to be a difficult one for sure – they are under pressure as well to win this game and it's going to make it an interesting match," said Di Matteo.
"It will be an open game. Both sides can win it. Juve are at home and maybe that could be a bit of an advantage, but sometimes playing at home can be a hindrance too.
"I think we've got to try and create problems for Juve. I've seen lots of their recent games, including the last one against Lazio, when they crushed their opponents in their own half. We have to cause them problems. Yes, we will defend, but not only that."
Juventus are currently third in Group E with six points from four matches, a point behind Chelsea, who are second.