Ronald Koeman's side will again be without the services of Philippe Coutinho, Ansu Fati and Sergi Roberto for Saturday's contest, and it is still unclear when each of the trio could return to the field.
Miralem Pjanic and Ronald Araujo remain doubts, meanwhile, with the former missing the last two matches with an ankle problem, while the latter was absent against Sevilla in the Copa del Rey on Wednesday, having picked up a problem on his return to the field against Sevilla in the league last weekend.
Koeman has plenty of decisions to make as Barca will face Paris Saint-Germain in the second leg of their last-16 Champions League clash next Wednesday, trailing 4-1 from the first leg.
Barca could revert to a back four, though, with Mingueza, as mentioned, coming in for the injured Pique, while Koeman is likely to make alterations in attack with Antoine Griezmann and Trincao potentially coming back in.
Ousmane Dembele was on the scoresheet in the impressive Copa del Rey turnaround against Sevilla but could be given the chance to rest ahead of the PSG fixture.
Pedri was only cleared to return from injury in the build-up to Wednesday's game, but the teenager is expected to again be named in the starting XI against Osasuna, who have won three of their last four.
Barcelona possible starting lineup: Ter Stegen; Dest, Mingueza, Lenglet, Alba; Pedri, Busquets, De Jong; Trincao, Messi, Griezmann