At the weekend, Messi was forced to withdraw from the squad to face Malaga due to the birth of his child, but the Argentine will return for the second leg of the Champions League last-16 tie at Camp Nou.
Rather than being distracted by life off the pitch, Busquets has predicted that Messi will be "even more motivated" to get on the scoresheet against the Premier League side.
At a press conference, the 29-year-old told reporters: "I think that he is going to be okay and even more motivated.
"Physically he is going to be great and be more relaxed. I hope he is going to be perfect and play like he usually plays."
Last month's first leg at Stamford Bridge ended in a 1-1 draw.