As promised, Arsene Wenger makes significant changes for the game, which represents the first time that the Gunners have played in the competition in 17 years.
In all, the Frenchman makes nine changes from the 3-0 victory over Bournemouth in the Premier League on Saturday, with only left-back Nacho Monreal and Hector Bellerin on the right wing keeping their places for the visit of the Bundesliga side.
Laurent Koscielny is suspended for the game, while Wenger has given Petr Cech, Alexandre Lacazette, Mesut Ozil, Danny Welbeck, Granit Xhaka and Aaron Ramsey the night off ahead of an important game with rivals Chelsea on Sunday.
Despite the plethora of changes, there is no start for Jack Wilshere, who is instead named on the bench as he gears up to make his first Arsenal appearance in 383 days.
For the visitors, Peter Stoger makes just two changes following on from the 3-0 defeat at Augsburg at the weekend - the side's third defeat from three Bundesliga outings this season.
Arsenal: Ospina; Holding, Mertesacker, Monreal; Bellerin, Iwobi, Elneny, Maitland-Niles; Walcott, Giroud, Sanchez
Subs: Macey, Mustafi, Kolasinac, Wilshere, Rene-Adelaide, Nelson, Akpom
FC Koln: Horn; Klunter, Jorge Mere, Heintz, Rausch; Lehmann; Zoller, Hoger, Hector, Bittencourt; Cordoba
Subs: Kessler, Sorensen, Risse, Jojic, Osako, Guirassy, J. Horn
Keep up with all of the action from the Emirates this evening with Sports Mole's live text commentary here.