The Gunners, who saw off Marseille 2-0 on Tuesday, only need to avoid a heavy defeat in Italy on December 11 to make it into the last 16 of the competition.
However, Flamini claims that he and his teammates will be wanting to win next month's match at the Stadio San Paolo.
"When you play for Arsenal and you have ambition, the objective is to win every game," he told the club's official website.
"Of course we'll go to Napoli and we'll go there to win the game, we'll go there to try our best and finish top of this group because we know it's important."
Arsenal continue their Premier League campaign on Saturday when they visit Cardiff City.