Centre-back Mats Hummels made his return from injury against Schalke, but is unlikely to start again in Brussels.
Victory at the Stade Constant Vanden Stock would put BVB in an excellent position in the group on six points and they will hope not to be the first German side to lose against Belgian opposition in the Champions League.
As for hosts Anderlecht, they go into the match top of the Belgian Pro League with 21 points from nine games.
They were on course to shock the Turkish side until an injury-time equaliser arrived from Burak Yilmaz.
Number one Silvio Proto is set to return from suspension and replace Davy Roef between the sticks, while 17-year-old Youri Tielemans, who was the youngest player in any Champions League team on matchday one, could again start in midfield.
If the Purple and White do cause an upset, it will end a run of nine games without a win in European competition.
Dortmund's only previous away trip to Anderlecht came in the 1990-91 UEFA Cup when the Belgian side came out on top 1-0.
Possible starting lineups
Anderlecht: Proto; Colin, Heylen, Mangulu, Deschacht; Najar, Tielemans, Defour, Acheampong; Praet; Suarez
Dortmund: Weidenfeller; Piszczek, Sokratis, Subotic, Durm; Ginter, Bender; Grosskreutz, Kagawa, Aubameyang; Immobile
Sports Mole says: 0-2