The Bianconeri suffered their ninth league defeat of the campaign after they were beaten 2-0 at home by Torino, and Guidolin said that he is willing to resign if their poor form is down to him.
"If I realise that I am the problem, then I am prepared to walk away. I have done it before in my career and am entirely ready to do so again, if needed," he told Sky Sport Italia.
"I care about Udinese too much, so I suffer too much for this team. Perhaps I transmit some of that suffering to the team and they subconsciously absorb it.
"If I realise that I am a problem rather than a resource for the club, then of course I would step aside. I haven't spoken to the club, I am just telling you this."
Udinese are currently 15th in the Serie A table.