A double from Paul Benson helped Swindon Town secure the League Two title with a 5-0 win over Port Vale at the County Ground.
Shrewsbury Town booked their spot alongside the Robins in League One for next season with a 1-0 win over Dagenham and Redbridge.
Crawley Town missed the opportunity to join them as they were surprisingly beaten 3-0 at home by Hereford Town, who maintained their League Two status for another week with the victory.
The Bulls will now fight it out with Barnet for Football League survival on the final day of fixtures after Martin Allen's side beat Wimbledon 4-0.
At the other end of the table, Macclesfield were condemned to the Conference for next season after losing 2-0 to Burton Albion.
Cheltenham Town secured their spot in the playoffs with a 3-1 win over Bradford City while Crewe Alexandra moved a step closer to joining them courtesy of Nick Powell's stoppage-time equaliser against Torquay United.
League Two results: Aldershot Town 2-2 Rotherham; Barnet 4-0 AFC Wimbledon; Bristol Rovers 5-1 Accrington Stanley; Cheltenham Town 3-1 Bradford City; Crawley Town 0-3 Hereford Town; Macclesfield Town 0-1 Burton Albion; Morecambe 2-2 Plymouth Argyle; Northampton Town 1-1 Gillingham Town; Oxford United 0-2 Southend United; Shrewsbury Town 1-0 Dagenham & Redbridge; Swindon Town 5-0 Port Vale; Torquay United 1-1 Crewe Alexandra.