The Premier League side make the trip to face APOEL on Tuesday night after registering a 3-1 victory over Borussia Dortmund on matchday one.
Pochettino is without half-a-dozen first-team players for the fixture against the group minnows, but the Argentine has suggested that the club cannot afford to drop points if they want to secure progress through to the last 16 after Christmas.
The 45-year-old told reporters: "The win against Dortmund was so important and it's key for our future in the Champions League to win tomorrow again.
"Of course we will miss the players who are not here, but it's about the collective and it's important to deal with it."
Spurs face back-to-back fixtures with Real Madrid after their meeting with APOEL.